5 alpha Chemically Competent Cell abx098065|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司

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產品名稱：5 alpha Chemically Competent Cell
產品型號：abx098065
產品廠商：abbexa
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5 alpha Chemically Competent Cell

詳情介紹：

5α Chemically Competent Cell is designed for chemical transformation of DNA, with blue/white selection, reduced recombinantion of cloned DNA, and a transformation efficiency of over 10⁸ cfu/µg DNA (tested by pUC19 plasmid DNA).

The genotype is: F^-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (r_k^-, m_k⁺) supE44λ- thi-1 gyrA96 relA1 phoA.

The 1 ml size consists of 10 × 100 µl Competent Cells, and 20 μl (0.1 ng/μl) Control Plasmid pUC19.

Target	5 alpha Chemically Competent Cell
Storage	Store at -70 °C for up to 6 months. Do not store in liquid nitrogen. Avoid repeated freeze/thaw cycles.
Availability	Shipped within 10-20 working days.
Note	This product is for research use only. This product is shipped with dry ice.
Directions for use	Recommended Protocol: Equilibrate a water bath to 42 °C. Bring a vial of SOC medium or LB medium to room temperature. Warm selective plates to 37 °C for 30 minutes. Thaw 100 µl of 5 alpha Chemically Competent Cell on ice. Aliquot 50 µl of cells into a pre-chilled 1.5 ml tube, then add target DNA (1-5 µl). Do not mix by pipetting up and down. Leave on ice for 30 minutes. Heat-shock the cells for 45 seconds at 42 °C, without shaking. Immediately transfer to the tube to ice. Leave on ice for 2 minutes without shaking. Add 500 µl of prewarmed SOC medium or LB medium (without antibiotics) into the tube. Mix well and shake at 37 °C for 1 hour at 200 RPM for cell recovery and expression of antibiotic resistance. Spread 20-200 µl from each transformation vial onto a pre-warmed selective plate. Cells that have not been plated can be stored at 4 °C and plated the next day if required. Invert the plates and incubate at 37 °C overnight. Select colonies and analyse by restriction enzyme digestion, PCR or sequencing. Note: Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. All samples and reagents require gentle handling throughout the entire procedure.

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產品名稱：5 alpha Chemically Competent Cell