產品詳情
簡單介紹：
Agarose Low EEO
詳情介紹：
The Low EEO agarose is the highest quality molecular biology grade agarose suitable for analytical and preparative electrophoresis of nucleic acids used in the separation of high molecular weight nucleic acids in low gel concentrations.
Specification:
| Moisture | ≤ 10% |
| Gel strength (1.5% gel) | ≥ 1300 g/cm2 |
| Gelling point (1.5% gel) | 41-45 °C |
| Melting point (1.5% gel) | 84-88 °C |
| Target | Agarose Low EEO |
| Storage | Store at room temperature. |
| Availability | Shipped within 10-15 working days. |
| Note | This product is for research use only. |
| Directions for use | Preparation of agarose gel in the microwave. 1. Define the quantity needed to prepare the gel at the desired concentration. 2. Add necessary volume of run buffer (TAE, TBE to 1X). 3. Homogenize and microwave for 2 - 4 minutes, considering cycles of 30 seconds, until the sample is completely dissolved. Caution: Use necessary protection for the handling of hot material. Suggestion: Work in microwave at medium power. 4. Add selected staining agent (e.g. Ethidium Bromide or other) and homogenize 5. Allow to cool to 60° C - at room temperature - load electrophoresis mold and let gel. 6. Remove mold from wells, mount electrophoresis chamber, load samples and run buffer - same buffer with which the gel was prepared - and start the electrophoretic run. Preparation agarose gel in heating plate. 1. Define the quantity needed to prepare the gel at the desired concentration. 2. Add necessary volume of run buffer (TAE, TBE to 1X). 3. Homogenize and carry out a heating plate with stirring. Once the boiling has started, measure 20 minutes (use a lid or refrigerant system to avoid concentrating the sample). 4. Add selected staining agent (e.g. Ethidium Bromide or other) and homogenize. 5. Allow to cool to 60° C - at room temperature - load electrophoresis mold and let gel. 6. Remove mold from wells, mount electrophoresis chamber, load samples and run buffer - same buffer with which the gel was prepared - and start the electrophoretic run. |
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