Human Apolipoprotein E/ApoE protein (Recombinant) (C-His) (STJP001435) STJP001435|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司

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產品名稱：Human Apolipoprotein E/ApoE protein (Recombinant) (C-His) (STJP001435)
產品型號：STJP001435
產品廠商：ST john's laboratory
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簡單介紹：

Human Apolipoprotein E/ApoE protein (Recombinant) (C-His) (STJP001435)

詳情介紹：

Host:	HEK293 cells
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Recombinant-Human Apolipoprotein E/ApoE-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications.

Formulation:

Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4.

Gene Symbol:	APOE
Gene ID:	348
Uniprot ID:	APOE_HUMAN
Immunogen:	Recombinant Human Apolipoprotein E/ApoE Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys19-His317) of human Apolipoprotein E/ApoE (Accession #NP_000032.1) fused with a 6×His tag at the C-termin

Post Translational Modifications	APOE exists as multiple glycosylated and sialylated glycoforms within cells and in plasma. The extent of glycosylation and sialylation are tissue and context specific. Plasma APOE undergoes desialylation and is less glycosylated and sialylated than the cellular form. Glycosylation is not required for proper expression and secretion. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 and Ser-314 are minor glycosylation sites compared to Ser-308. Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold). Phosphorylated by FAM20C in the extracellular medium. Undergoes C-terminal proteolytic processing in neurons. C-terminally truncated APOE has a tendency to form neurotoxic intracellular neurofibrillary tangle-like inclusions in neurons.
Function	APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosesynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting. APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells. Binds to the immune cell receptor LILRB4. APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP. (Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles. This interaction is probably promoted via the up-regulation of cellular autophagy by the virus.
Protein Name	Apolipoprotein E Apo-E
Database Links	Reactome: R-HSA-1251985 Reactome: R-HSA-3000480 Reactome: R-HSA-381426 Reactome: R-HSA-8864260 Reactome: R-HSA-8957275 Reactome: R-HSA-8963888 Reactome: R-HSA-8963901 Reactome: R-HSA-8964026 Reactome: R-HSA-8964058 Reactome: R-HSA-9029569 Reactome: R-HSA-975634 Reactome: R-HSA-977225
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Extracellular Vesicle Endosome Multivesicular Body In The Plasma Apoe Is Associated With Chylomicrons Chylomicrons Remnants Vldl Ldl And Hdl Lipoproteins Lipid Poor Oligomeric Apoe Is Associated With The Extracellular Matrix In A Calcium- And Heparan-Sulfate Proteoglycans-Dependent Manner Lipidation Induces The Release From The Extracellular Matrix Colocalizes With Cd63 And Pmel At Exosomes And In Intraluminal Vesicles Within Multivesicular Endosomes
Alternative Protein Names	Apolipoprotein E protein Apo-E protein APOE protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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產品名稱：Human Apolipoprotein E/ApoE protein (Recombinant) (C-His) (STJP001435)