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  • 产品名称：Human IGFBP1 (Insulin-like growth factor-binding protein 1) CLIA Kit (STJC0000259)

  • 产品型号：STJC0000259
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IGFBP1 (Insulin-like growth factor-binding protein 1) CLIA Kit (STJC0000259)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human IGFBP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IGFBP1
Gene ID: 3484
Uniprot ID: IBP1_HUMAN
Specificity: This kit recognizes Human IGFBP-1 in samples. No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated.probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Protein Name Insulin-Like Growth Factor-Binding Protein 1
Ibp-1
Igf-Binding Protein 1
Igfbp-1
Placental Protein 12
Pp12
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Alternative CLIA Names Insulin-Like Growth Factor-Binding Protein 1 CLIA kit
Ibp-1 CLIA kit
Igf-Binding Protein 1 CLIA kit
Igfbp-1 CLIA kit
Placental Protein 12 CLIA kit
Pp12 CLIA kit
IGFBP1 CLIA kit
IBP1 CLIA kit
Specificity This kit recognizes Human IGFBP-1 in samples. No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-107 97
EDTA plasma(n=8) 92-106 97
Cell culture media(n=8) 94-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.38 194.91 799.38 104.45 186.91 801.01
Standard deviation 8.31 21.48 87.13 9.67 15.14 84.35
CV (%) 8.28 11.02 10.90 9.26 8.10 10.53
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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