产品详情

  • 产品名称：Human HPSE (Heparanase) Sandwich ELISA (STJE0003651)

  • 产品型号：STJE0003651
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human HPSE (Heparanase) Sandwich ELISA (STJE0003651)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This HPSE Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: HPSE
Gene ID: 10855
Uniprot ID: HPSE_HUMAN
Specificity: This kit recognizes Human HPA in samples. No significant cross-reactivity or interference between Human HPA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme. N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
Function Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Protein Name Heparanase
Endo-Glucoronidase
Heparanase-1
Hpa1 Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-6798695
Cellular Localisation Lysosome Membrane
Peripheral Membrane Protein
Secreted
Nucleus
Proheparanase Is Secreted Via Vesicles Of The Golgi
Interacts With Cell Membrane Heparan Sulfate Proteoglycans (Hspgs)
Endocytosed And Accumulates In Endosomes
Transferred To Lysosomes Where It Is Proteolytically Cleaved To Produce The Active Enzyme
Under Certain Stimuli
Transferred To The Cell Surface
Associates With Lipid Rafts
Colocalizes With Sdc1 In Endosomal/Lysosomal Vesicles
Accumulates In Perinuclear Lysosomal Vesicles
Heparin Retains Proheparanase In The Extracellular Medium
Alternative ELISA Names Heparanase ELISA kit
Endo-Glucoronidase ELISA kit
Heparanase-1 ELISA kit
Hpa1 Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit ELISA kit
HPSE ELISA kit
HEP ELISA kit
HPA ELISA kit
HPA1 ELISA kit
HPR1 ELISA kit
HPSE1 ELISA kit
HSE1 ELISA kit
Specificity This kit recognizes Human HPA in samples. No significant cross-reactivity or interference between Human HPA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 98
EDTA plasma (n=8) 87-102 94
Cell culture media (n=8) 92-106 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.54 1.09 4.17 0.53 1.15 4.57
Standard deviation 0.04 0.05 0.13 0.04 0.05 0.19
CV (%) 7.41 4.59 3.12 7.55 4.35 4.16
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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