產品詳情
簡單介紹：
Mouse CYP7A1 (Cytochrome P450 7A1) CLIA Kit (STJC0001187)
詳情介紹：
| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description: | This mouse CYP7A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Cyp7a1 |
| Gene ID: | 13122 |
| Uniprot ID: | CP7A1_MOUSE |
| Specificity: | This kit recognizes Mouse CYP7A1 in samples. No significant cross-reactivity or interference between Mouse CYP7A1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosesynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosesynthesis (Probable). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives. |
| Protein Name | Cytochrome P450 7a1 24-Hydroxycholesterol 7-Alpha-Hydroxylase Cypvii Cholesterol 7-Alpha-Hydroxylase Cholesterol 7-Alpha-Monooxygenase |
| Database Links | Reactome: R-MMU-192105 Reactome: -MMU-193368 Reactome: -MMU-193807 Reactome: -MMU-211976 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Microsome Membrane |
| Alternative CLIA Names | Cytochrome P450 7a1 CLIA kit 24-Hydroxycholesterol 7-Alpha-Hydroxylase CLIA kit Cypvii CLIA kit Cholesterol 7-Alpha-Hydroxylase CLIA kit Cholesterol 7-Alpha-Monooxygenase CLIA kit Cyp7a1 CLIA kit Cyp7 CLIA kit |
| Specificity | This kit recognizes Mouse CYP7A1 in samples. No significant cross-reactivity or interference between Mouse CYP7A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 96-111 | 101 |
| EDTA plasma(n=8) | 91-105 | 97 |
| Cell culture media(n=8) | 85-99 | 92 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 210.39 | 483.68 | 1361.79 | 192.11 | 530.62 | 1442.14 |
| Standard deviation | 23.44 | 50.50 | 137.40 | 21.63 | 44.73 | 130.08 |
| CV (%) | 11.14 | 10.44 | 10.09 | 11.26 | 8.43 | 9.02 |
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