產品詳情
簡單介紹：
Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) Sandwich ELISA (STJE0019734)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25-2000pg/mL |
| Short Description: | This ICAM-1/CD54 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Icam1 |
| Gene ID: | 15894 |
| Uniprot ID: | ICAM1_MOUSE |
| Specificity: | This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Protein Name | Intercellular Adhesion Molecule 1 Icam-1 Mala-2 Myd10 Cd Antigen Cd54 |
| Database Links | Reactome: R-MMU-198933 Reactome: -MMU-216083 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein |
| Alternative ELISA Names | Intercellular Adhesion Molecule 1 ELISA kit Icam-1 ELISA kit Mala-2 ELISA kit Myd10 ELISA kit Cd Antigen Cd54 ELISA kit Icam1 ELISA kit Icam-1 ELISA kit |
| Specificity | This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 88-100 | 93 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 94-108 | 101 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.43 | 196.42 | 853.47 | 96.67 | 202.08 | 925.10 |
| Standard deviation | 6.68 | 8.78 | 40.37 | 6.24 | 11.88 | 41.54 |
| CV (%) | 6.46 | 4.47 | 4.73 | 6.46 | 5.88 | 4.49 |
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