產品詳情
簡單介紹：
Mouse IGF-1 (Insulin-like Growth Factor 1) Sandwich ELISA (STJE0019727)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.75ng/mL |
| Detection Limit: | 1.25-80pg/mL |
| Short Description: | This IGF-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Igf1 |
| Gene ID: | 16000 |
| Uniprot ID: | IGF1_MOUSE |
| Specificity: | This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Protein Name | Insulin-Like Growth Factor I Igf-I Somatomedin |
| Database Links | Reactome: R-MMU-114608 Reactome: -MMU-2404192 Reactome: -MMU-2428928 Reactome: -MMU-2428933 Reactome: -MMU-381426 Reactome: -MMU-422085 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor I ELISA kit Igf-I ELISA kit Somatomedin ELISA kit Igf1 ELISA kit Igf-1 ELISA kit |
| Specificity | This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 87-99 | 93 |
| EDTA plasma (n=8) | 94-110 | 101 |
| Cell culture media (n=8) | 94-107 | 99 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.31 | 12.76 | 30.55 | 4.06 | 12.92 | 30.23 |
| Standard deviation | 0.27 | 0.65 | 1.15 | 0.26 | 0.57 | 1.25 |
| CV (%) | 6.20 | 5.10 | 3.78 | 6.52 | 4.42 | 4.13 |
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