產品詳情
簡單介紹：
Mouse PF4 (Platelet Factor 4) Sandwich ELISA (STJE0019780)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.38ng/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This PF4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Pf4 |
| Gene ID: | 56744 |
| Uniprot ID: | PLF4_MOUSE |
| Specificity: | This kit recognizes Mouse PF4 in samples. No significant cross-reactivity or interference between Mouse PF4 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation. |
| Protein Name | Platelet Factor 4 Pf-4 C-X-C Motif Chemokine 4 |
| Database Links | Reactome: R-MMU-114608 Reactome: -MMU-140875 Reactome: -MMU-202733 Reactome: -MMU-380108 Reactome: -MMU-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Platelet Factor 4 ELISA kit Pf-4 ELISA kit C-X-C Motif Chemokine 4 ELISA kit Pf4 ELISA kit Cxcl4 ELISA kit Scyb4 ELISA kit |
| Specificity | This kit recognizes Mouse PF4 in samples. No significant cross-reactivity or interference between Mouse PF4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 88-101 | 95 |
| EDTA plasma (n=8) | 84-99 | 91 |
| Cell culture media (n=8) | 84-98 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.27 | 312.54 | 739.29 | 111.53 | 312.50 | 747.32 |
| Standard deviation | 7.20 | 13.69 | 32.23 | 5.75 | 16.09 | 36.54 |
| CV (%) | 6.97 | 4.38 | 4.36 | 5.16 | 5.15 | 4.89 |
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