Mouse TFR1 (Transferrin Receptor 1) Sandwich ELISA (STJE0005068) STJE0005068|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司

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產品名稱：Mouse TFR1 (Transferrin Receptor 1) Sandwich ELISA (STJE0005068)
產品型號：STJE0005068
產品廠商：ST john's laboratory
產品文檔：

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Mouse TFR1 (Transferrin Receptor 1) Sandwich ELISA (STJE0005068)

詳情介紹：

Applications:	ELISA
Reactivity:	Mouse
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.38ng/mL
Detection Limit:	0.63~40ng/mL
Short Description:	This TFR1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Tfrc
Gene ID:	22042
Uniprot ID:	TFR1_MOUSE
Specificity:	This kit recognizes Mouse TFR1 in samples. No significant cross-reactivity or interference between Mouse TFR1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Upon stimulation, positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.
Protein Name	Transferrin Receptor Protein 1 Tr Tfr Tfr1 Trfr Cd Antigen Cd71
Database Links	Reactome: R-MMU-432722 Reactome: -MMU-8856825 Reactome: -MMU-8856828 Reactome: -MMU-8980692 Reactome: -MMU-9013026 Reactome: -MMU-9013106 Reactome: -MMU-9013149 Reactome: -MMU-9013404 Reactome: -MMU-9013406 Reactome: -MMU-9013407 Reactome: -MMU-9013408 Reactome: -MMU-9013423 Reactome: -MMU-917977 Reactome: -MMU-9696270 Reactome: -MMU-9696273
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Melanosome
Alternative ELISA Names	Transferrin Receptor Protein 1 ELISA kit Tr ELISA kit Tfr ELISA kit Tfr1 ELISA kit Trfr ELISA kit Cd Antigen Cd71 ELISA kit Tfrc ELISA kit Trfr ELISA kit
Specificity	This kit recognizes Mouse TFR1 in samples. No significant cross-reactivity or interference between Mouse TFR1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	89-102	94
EDTA plasma (n=8)	95-106	101
Cell culture media (n=8)	85-97	91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.84	3.55	18.61	1.66	3.50	19.61
Standard deviation	0.10	0.17	0.78	0.09	0.18	1.07
CV (%)	5.43	4.79	4.19	5.42	5.14	5.46

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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產品名稱：Mouse TFR1 (Transferrin Receptor 1) Sandwich ELISA (STJE0005068)